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Scientist- Protein Engineering

Fulltime/ Onsite

Bangalore, Karnataka, India

Full time/ Onsite

About the Role

Biolockey Healthworks is currently seeking a highly motivated and skilled individual to fill the role of Scientist-Protein Engineering within our dynamic team. The position is available from 1st May, 2024.

We are looking for a highly motivated, independent, and meticulous protein biologist with experience in handling multiple expression systems. Person should be ready to work flexibly
and cohesively across teams.


  • MSc/ MTech degree with 5-8+ years of relevant industry experience OR PhD in the field of Biochemistry, Structural Biology, biophysics. Candidate must have 3-4 years of experience in the area of molecular biology and protein expression either in academia/ biopharma or biotechnology industry setting.

  • Demonstrated expertise in the detailed analytical characterization of proteins using analytical chromatography, capillary electrophoresis, light scattering, differential scanning fluorimetry, mass spectrometry, Bio-layer interferometry, FACS or related techniques

  • Ability to analyze large data sets and interpret and communicate data. The scientist will be responsible for strategic experimental planning, execution, reporting results, and requisite documentation.

  • Strong interpersonal skills with the ability to work collaboratively as a member of a cross-functional team

  • Familiarity with Nanobody binding and other mechanistic data from SPR or related technologies is an advantage


  • Developing high-throughput workflows for analytical and biophysical screening of Nanobody properties

  • Optimizing and operating diverse analytical and biophysical instrumentation such as analytical chromatography (HPLC), capillary electrophoresis (CE), light scattering (DLS), cell-sorting (FACS). At least 3-4 years of experience in protein purification using various chromatographic methods. Proficiency in running protein purifications using Akta and Agilent systems.

  • Cloning and expression in E. coli and yeast systems – clone generation and assessment in shake flasks, Clone characterization, evolution of expression systems. Experience in recombinant protein expression in bioreactors will be considered an additional advantage.

  • Perform biofunctionalization reactions, immobilize proteins and DNA, PCR, RT-PCR, proteomic assays

  • · Design assays to validate and characterize activities of Nanobodies, detect protein interactions with sensors such as surface plasmon resonance (SPR)

  • Design immunoassays, including sandwich ELISA to quantitate nanobodies and cellular metabolites, membrane-bound and intracellular proteins

  • Analyzing and assembling large dataset of detailed Nanobody characterization including analytics, biophysics and in silico assessment

  • Support lead panel selection using analytical, biophysical and binding data

  • Training and mentoring of junior team members of a dynamic team. Managing timely delivery of project milestones and providing regular updates to BHPL Integrity, transparency, innovation, and collaboration are values driving BioLOCKEY team and is expected in all incoming members.

  • Collaborating with teammates from discovery biology, protein engineering and other teams to build platforms and processes, as well as analyze, interpret and communicate data

Initial 6 months on probation and extension on performance

Commensurate with experience


Aspirants possessing requisite qualifications may apply with their detailed CV (including names of two references) to

Candidates selected after initial screening will be interviewed.

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